Alexa phalloidin 488
WebJul 1, 2024 · Phalloidin-iFluor ® 488 conjugate PBS 3.0 - 4.0% Methanol-free formaldehyde 0.1% Triton X-100 BSA Cell culture or tissue sample for staining Optional: ethanolamine Optional: FluoroQuest Mounting Medium with DAPI Actin Staining Protocol Note: If using paraffin-embedded tissue samples, a deparaffinization and rehydration step is required. WebProduct overview. Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be …
Alexa phalloidin 488
Did you know?
WebAdd 1 µL of AF488 Phalloidin solution to 1 mL of PBS with 1% BSA. N o te The stock solution of phalloidin conjugate should be aliquoted and stored at -20 °C. protected from light. N o te Different cell types might be stained differently. The concentration of phalloidin conjugate working solution should be prepared accordingly. WebMar 30, 2024 · For actin staining, Alexa Fluor 488 phalloidin (Molecular Probes, 1 μL per 22 × 22 mm coverslip) was added to the secondary antibody step. All coverslips were then mounted onto Mowiol supplemented with 0.1 μg/mL DAPI (Sigma-Aldrich). Cells were observed on confocal microscope (Leica TCS SP8) with HC PL APO 63×/1.40 oil …
WebAdd XFD488 Phalloidin Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under … WebFeb 14, 2024 · The specimens were labeled with Alexa Fluor 594 phalloidin, and rabbit antibodies against WBNV N protein followed by FITC-conjugated goat anti-rabbit antibody. Alexa Fluor 594- and FITC-specific fluorescence signals were captured by confocal microscopy. (A). Alexa Fluor 594 phalloidin-conjugated F-actin probe emitted red …
WebActinGreen 488 reagent is formulated as a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) and comes in a convenient dropper bottle. Just tip and … WebMar 30, 2024 · Ruan Y, Zhou J, Kang N, Reed-Maldonado AB, Tamaddon A, Wang B, Wang HS, Wang G, Banie L, Lin G, Liu J, Lue TF. The effect of low-intensity extracorporeal shockwave therapy in an obesity-associated erectile dysfunction rat model. BJU Int. 2024 Jul;122(1):133-142. doi: 10.1111/bju.14202. Epub 2024 Apr 17.
WebAlexa Fluor 488® phalloidinis a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F …
WebAlexa Fluor Hg‐Link dye is a new generation of fluorescent probes that exhibit some advantages over other fluorescent dyes, such as being highly fluorescent over a broad pH range (pH 4 to 10), good water solubility, and higher photostability, allowing more time for … high school dxd ภาค 3 พากย์ไทย facebookWebDec 24, 2024 · For phalloidin staining, samples were incubated for 30 min with Alexa Fluor™ 568 Phalloidin Kit (Invitrogen™ through Thermo Fisher Scientific, Loughborough, UK). All samples were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) using NucBlue™ (Invitrogen™ through Thermo Fisher Scientific, Loughborough, UK). high school dxd youtubeWebHuman peripheral mononuclear cell and neutrophil mixed cells were fixed with 2% paraformaldehyde (PFA) and then stained with 5 µg/ml CD11b (clone ICRF44) Alexa Fluor® 594 (red) and 5 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI and are shown in blue. high school dxd ภาค 3WebActi-stain™ 488 phalloidin-labeled actin filaments retain many functional characteristics of unlabeled actin including their ability to interact with myosin. Actin-stain™ 488 phalloidin is supplied as an orange solid. … how many chapters are in chainsaw man mangaWebSix individual channels are captured via staining with Hoechst 33342 (channel 1, blue), Alexa Fluor 488 Concanavalin A (channel 2, green), Alexa Fluor 568 Phalloidin (channel 3, red), Syto14 (channel 4, cyan), MitoTracker Deep Red FM (channel 5, magenta), Alexa Fluor 555 Agglutinin (channel 6, yellow). how many chapters are in chainsaw man vol 1WebAlexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Invitrogen™ Alexa … high school dxd ภาค 4WebFinally, the cells were stained with Alexa Phalloidin 555, to bind the actin filaments and highlight the cell morphology. ... (488 nm). The zeta potential of the hybrid Rh-CQDs NPs was estimated as −28 ± 1 mV, revealing the presence of carboxyl groups on the surface of the hybrid NPs, even after the conjugation process. Moreover, the ... how many chapters are in dead space 3