WebDec 15, 2024 · Both systems are equipped with a Smartbeam II UV laser (355 nm) operating at a repetition rate of 1000 Hz. For the method optimization for FT-MS, we set the lock mass on m/z 500–800. All spectra were normalized by the total ion current. We set the same spatial resolution with 150 μm and mass range of m/z 400–1500. Websectioned mineralized using Cryofilm type IIC(10) (Section-Lab Co., Hiroshima, Japan). Coronal sections (5 µm) were cut to assess the proximal metaphysis. Images were cap-tured using Aperio Scanscope FL and Aperio Imagescope v11.2.0.780 (Aperio, Vista, CA, USA). A cancellous ROI of 1.2 mm height, commencing 0.5 mm below the proximal
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WebCryofilm type 3c (SECTION-LAB, Japan). For immunofluorescent immunohistochemistry, sections were washed in 1× PBS, blocked in 5% normal goat serum (S-1000, Vector Labs, CA, USA) for 30 min, and ... 1 9. The lower region of the IFP was defined as the perivascular area. 19 . All quantifications were based on random 10x field images. WebCryofilm type 3c (SECTION-LAB, Japan). For immunofluorescent immunohistochemistry, sections were washed in 1× PBS, blocked in 5% normal goat serum (S-1000, Vector … ford powerstroke super duty
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