Pshuttle-gfp
WebProduct Specs. Item pShuttle-IRES-hrGFP-2. Company Agilent Technologies. Price Supplier Page View Company Product Page. Catalog Number 240082. Type Adenoviral Vector. … WebMethods: The pSport 1-mSD274 plasmid containing the full-length PD-L1 cDNA of the mouse was digested and subcloned to the shuttle plasmid pShuttle-GFP-CMV (-), and then the adenovirus skeleton plasmid pAdxsi-GFP-CMV-PD-L1 was constructed by enzymolysis and ligation, transformed into DH5α sensitive bacteria, and screened for positive clones.
Pshuttle-gfp
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Webtor pShuttle-CMV-EGFP. The plasmid pShuttle-CMV-EGFP was linearized with EcoRV and Bg1II, and was afterward dephosphorylated with alkaline phosphatase (CIAP), which was then ligated to a rHSG gene segment by T4 DNA ligase. The linearized pShuttle-GFP-rHSG was transformed into the competent BJ5183 strain which was transformed with pAdxsi. WebpShuttle-IRES-hrGFP-1 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially …
WebMar 3, 2016 · Recombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, … WebDec 15, 2024 · In Brief, pShuttle-GFP-Gas6 was constructed by cloning the target gene Gas6 into pShuttle-GFP-CMV. Then, the pShuttle-GFP-Gas6 was transferred into the …
WebJul 24, 2007 · TrkA-PC12 cells were transfected with a cDNA for GFP-tagged Rab7 (Rab7-GFP) and Pincher-HA. In these cells, P-Trk and Rab7-GFP were rarely colocalized in cytoplasmic structures (weighted colocalization coefficient for Rab7-GFP = 0.16 ± 0.14; P-TrkA = 0.11 ± 0.19; n = 17), even after 1 h of NGF treatment ( Fig. 4 C Upper ). WebRecombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or …
WebHGF gene or GFP gene was amplified by PCR and inserted into the pShuttle-CMV (Stratagene, LaJolla, CA, USA), obtaining the pShuttle plasmid pShuttle-HGF or pShuttle-GFP. pShuttle-HGF and pShuttle-GFP were then used to generate Ad-HGF and Ad-GFP, respectively, ac-cording to the manual accompanying the AdEasy system.
Webwith green fluorescence protein (GFP) gene. The PDX1 gene from pShuttle-GFP-CMV-PDX1 was transferred into adenoviral vector (pAdxsi) (Gibco-Invitrogen) and linearized with I-CeuI + I-SceI enzyme. All sequences were confirmed by DNA sequencing. The confirmed recombinant adenoviral Differentiation of PDX1 gene-modified human umbilical cord oze college victor hugo la celle saint cloudWebNov 2, 2015 · The Ad vector pShuttle-CMV (Stratagene) was rendered replication defective by deletion of the E1 and E 3 genes [ 23 ]. The GFP coding sequence was amplified from the pEGFP-N1 vector (Clontech) using the primer pair 5′-ATGGTGAGCAAGGGCGAG-3′ and 5′-TTACTTGTACAGCTCGTCC-3′. oze di attualità italianeWebVector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired. Max insert size = 7.5kb with pAdEasy1 and 10.2kb with … oze college renoir chatouWebpSuper pSuper retro GFP-neo pSupeior puro pBabe puro pCMV-Gag-pol pCMV-VSV-G pCL Ampho pSuper retro puro pLVX-shRNA1 pLVX-shRNA2 pSicoR pSicoR pGK puro pLentilox 3.7 pLKO.1 puro PLKO.1 pLKO.3G pTet-PLKO-puro pPRIME-TET-GFP-FF3 pPRIME-TREX-GFP-FF3 pSIH1-H1-CoGFP pGIPZ empty pGIPZ control pTRIPZ empty pTRIPZ control pLVX … oze ecole nouvelle amsterdamWebA GGGGCC hexanucleotide repeat expansion in intron 1 of chromosome 9 open reading frame 72 (C9ORF72) gene is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal ... oze collège le racinayWebpShuttle-IRES-hrGFP-2. Plasmid type:Adenovirus systemCopy Number:Low copyPromoter:CMVCloning Method:Multiple cloning sites,restriction … oze college youri gagarineWebpShuttle-IRES-hrGFP-1. Cat.No. OVT2837. Host Cell. Mammalian cells, Escherichia coli. Product Type. Vector. Promoter. ozee dil dosti do